ANTI-INFLAMMATORY EFFECTS OF OZONE IN HUMAN MELANOMA CELLS AND ITS MODULATION OF TUMOUR MICROENVIRONMENT.

Ozone therapy is an effectivemedical treatment for different diseases like mucositis, psoriasis, acute pain,neurovascular diseases and cancer. Emerging evidence indicates that ozone, astrong oxidant, could effectively improve organ ischemia-reperfusion, herniateddisks and skin ulcers in clinical model with interesting anti-inflammatory properties through inhibition of NF-κB activation in acute and chronic disease.The aim of this study is based on the study of the biological effects of ozonein human melanoma cancer cells in order to investigate about its possible usein association to common therapy. Specifically, human melanoma cells were preexposed or not to pro-inflammatory condition (Lipopolysaccharides) andadministration of ozone at different concentration was performed in order toevaluate different  biological parameters; cell viability, Measurement ofMitochondrial Matrix Potential (MMP), Evaluation of p65-Nfkb and changes insecretion of interleukins and growth factors involved in melanoma growth,survival and chemo-resistance: IL-1, IL-8, IL-6, TNF-α, IL-9, TGF-β, IL-19,VEGF, MMP-2, MMP-9 and IL-17 by ELISA methods. Results obtained shown abilityof ozone to decrease cell viability up to 75% compared to control after 24h ofincubation and inhibit all interleukins analyzed and involved in melanoma cellsurvival and drug resistance. Ozone at 50 µg/ml also decrease of 60% theactivation of the pro-inflammatory mediator p65Nfkb and inhibit the Nitricoxide  production under pro-inflammatory condition. However, taking theseveral limitations of this study, further biological preclinicalinvestigations are being carried out in order to understand the potential ofozone as possible adjuvant agent in therapy of melanoma.


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